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. 2017 Sep 11;5(5):e00358. doi: 10.1002/prp2.358

Figure 6.

Figure 6

Coronary arterial segments cultured for 24 h in 1 nmol/L 17βΕ show attenuated contractions to Cav1.2 channel activation by 3 μmol/L FPL64176. Endothelium intact (+Endo) and denuded (‐Endo) arteries were cultured in 1 nmol/L 17βΕ or its solvent (EtOH). A, Experimental protocol used to evaluate the contractile response to the Cav1.2 channel agonist, FPL64176 (3 μmol/L). To prevent a potentially confounding influence of FPL‐induced Ca2+ release from sarcoplasmic reticulum (SR), rings initially were exposed to acetylcholine (Ach, 10 μmol/L) and thapsigargin (TSG, 10 μmol/L) before evaluating the contractile response to FPL64176. Complete relaxation of FPL64176‐induced contractions by 10 μmol/L nifedipine confirmed dependence on Cav1.2 channels. (B) Composite data from studies depicted in panel A. Data are expressed as mean ± S.E. and analyzed using a two‐way ANOVA (without replication) followed by Tukey HSD post hoc analysis. * P < 0.05, EtOH ± Endo versus 17βΕ ± Endo; (n = 7 pigs).