Figure 5.
Measuring cytotoxicity for the three‐ and four‐inhibitor combinations at subcytotoxic concentrations. Three‐ and four‐inhibitor combinations were prepared at CC 10 concentrations. A range of cytotoxicity assays including standard MTT, GSH, ATP, and trypan blue exclusion assays were conducted on HepG2 cells. APCE corresponds to actinomycin D, puromycin, cycloheximide, and emetine; ACE to actinomycin D, cycloheximide, and emetine; APE to actinomycin D, puromycin, and emetine; APC to actinomycin D, puromycin, and cycloheximide; and PCE to puromycin, cycloheximide; and emetine. Data are shown as mean ± SD from n = 3 independent experiments.