Fig. 2.

Assessment of activity of the IFN-α pathway in hY3 macrophage supernatants (Mac Sups). To authenticate the presence of IFN-α in hY3 macrophage supernatants, WISH cells were incubated without treatment, with macrophage supernatants, and with hY3-treated macrophage supernatants overnight. RNA was isolated from WISH cells, and an evaluation of the transcripts IFN-induced protein with tetratricopeptide repeats 1 (IFIT1) was performed. Values on the y-axis are the log₂-transformed values of $2^{-\mathrm{\Delta \Delta }{\text{C}}_{\text{T}}}$ (where C_T is threshold cycle); the x-axis shows the treatment of WISH cells. Values appearing above the dashed line represent IFIT1 levels greater than means + 2SD. The results showed that IFIT1 was highly abundant within the transcriptome reported by WISH cells given hY3 macrophage supernatants but not untreated macrophage supernatants (P = 0.02, Fisher’s exact test).