Figure 3.

NF-κB signaling augments IL-4 induction of IL-17Rb. (A) Treatment with IL-25 activates IκB kinase (IKK). (B) The IκB inhibitor of NF-κB is phosphorylated and degraded after IL-4 treatment (50 ng/ml). (C and D) A 30-min pretreatment of cells with inhibitors of STAT6 (AS1517499) or NF-κB (Bay-11–0782), each at 10 μM, prevented the induction of IL-17Rb mRNA (C) and protein (D). Blots are representative of three experiments performed on different days, and the graph represents an aggregate of three experiments with *P < 0.05 or **P < 0.01 by ANOVA against untreated cells (utx) and other 4-h conditions. Inh, inhibitor; NF-κB, nuclear factor κ B.