TABLE 2 .

A list of parameters used to build the cellular disposition model for MMAE and T-vc-MMAE

Parameters	Description	Units	Value (CV%)	Source
,	Volume of the media compartment for MMAE and T-vc-MMAE model, respectively	ml	3, 10	Fixed
	Scaling factor to convert the number of molecules to nanomoles.	Unitless		Fixed
,	Volume of each cell	pl	3.12, 8.14	Fixed
,	Doubling time associated with each cell line	H	40.1, 33.6	Singh et al. (2016b)
	Total concentration of intracellular tubulin	nM	65	Shah et al. (2012)
,	Second-order association and first-order dissociation rates of MMAE binding to tubulin	1/nM/h, 1/h	0.0183, 0.545	Shah et al. (2012)
,	Second-order association and first-order dissociation rates of T-vc-MMAE binding to HER2	1/nM/h, 1/h	0.37, 0.014	Maass et al. (2016)
	First-order net antibody-HER2-complex internalization rate	1/h	0.11	Maass et al. (2016)
	First-order nonspecific deconjugation rate of MMAE from ADC	1/h	∼0	Estimated to be very low value
	Average drug/antibody ratio for the formulation of T-vc-MMAE	Unitless	4.5	Measured in-house
	Number of HER2 receptors on N87 and GFP-MCF7 cells, respectively	Unitless	950,000, 52,000	Measured in-house
,	First-order rate of protease-induced intracellular ADC degradation and MMAE release	1/h	0.353 (9%), 0.03	Estimated, Maass et al. (2016)
	Average first-order influx rate constant for MMAE from extracellular space to intracellular space for N87 and MCF7 cells.	1/h	8.33 (8.5%)	Estimated
	Average first-order efflux rate constants for MMAE from intracellular space to extracellular space for N87 and MCF7 cells.	1/h	0.199 (14%)	Estimated