Fig. 1.
In vitro efficacy of ponatinib. Growth curve showing effect of various concentrations of ponatinib on GBM6 cells in vitro as determined by CyQUANT assay. The IC50 value resulting from response curves for the experimental total drug concentration (solid line, presented as the mean ± S.E.M; N = 3 in triplicate) or free drug concentration (dotted line, determined using the measured free fraction in serum-containing media). The total and free IC50 values were 1.08 and 0.032 µM, respectively, in GBM6 cell culture.