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Free fraction (fu) values determined from in vitro RED experiment after 4-hour incubation in triplicate. Two-sample t test was performed to compare plasma versus normal brain (*P < 0.05), normal brain versus BAT (P > 0.05; N.S., not significant), intracranial tumor core (IC tumor core) versus BAT (*P < 0.05), and IC core versus flank tumor (P > 0.05). Data are presented as the mean ± S.D.
