Fig. 9.

Inhibition of luciferase activity production at 4 °C or 37 °C by endocytic pathway inhibitors upon 2′-OMeUtaPS-mediated internalization of PMO sequence 10 in both serum-containing and serum-free media. For each experiment, the extracellular concentrations of 2′-OMeUtaPS and sequence 10 are 2.0 µM and 1.0 µM, respectively, whereas the extracellular concentrations of nystatin, latrunculin, cytochalasin D, chlorpromazine and EIPA are 30 µM, 2 µM, 2 µM, 50 µM and 0.5 mM, respectively. Error bars represent the mean ± SD of three independent experiments. EIPA, 5-(N-ethyl-N-isopropyl)amiloride; RLU, relative light units; FBS, fetal bovine serum.