TABLE 6.

Primers used in PCR amplification of specific B. burgdorferi and E. coli genes

Gene	Sequencea
	5′ oligonucleotide primer	3′ oligonucleotide primer
dbpAb	GGATCCCTCGAGATGATTAAATGTAATAATAAA	AAGCTTCTGCAGGTTATTTTTGCATTTTTCATC
ospCb	GGATCCCTCGAGATGAAAAAGAATACATTAAGT	AAGCTTCTGCAGAGGTTTTTTTGGACTTTCTGC
vlsEc	CTCGAGCTGCAGATGAAGAAGGATGATCAGATT	GAATTCGGATCCACCATCCTTCACAGCAAACTT
MBP gened	CTGCAGCTCGAGATGAAAATCGAAGAAGGTAAA	GAATTCCTGCAGCAGATCTTTGTTATAAATCTG

^aUnderlined regions represent the gene-specific sequences.

^bB. burgdorferi gene.

^cIR6 region of the B. burgdorferi vlsE gene.

^dMBP, E. coli maltose binding protein.