FIG 9.

Examination of transcription and replication efficiencies of Nishi-ΔL/Nluc in wtL- and mtL1929-33-expressing NA cells by real-time RT-PCR. NA cells were transfected to express wtL or mtL1929-33 before inoculation with Nishi-ΔL/Nluc at an MOI of 0.01. At 9 hpi (A and B) or 48 hpi (C, D, and E), the infected cells were collected and used for RNA extraction. The expression levels of N mRNA (A and C) and genomic RNA (B and D) in each cell lysate were measured by real-time RT-PCR. The levels are the number of copies of the respective RNAs per copy of mouse 18S rRNA. (E) The ratios of expression level of N mRNA to the level of genomic RNA were determined on the basis of their expression levels at 48 hpi. All assays were carried out in triplicate, and the values are means ± standard errors of the means. *, significant difference at a P value of <0.05; **, significant difference at a P value of < 0.01. ns, not significant (P ≥ 0.05).