FIG 2.

Analysis of sequence variants. The tax sequences obtained from NGS, Sanger sequencing of tax amplified from T cell lines, and Sanger sequencing of tax amplified from PBMC were analyzed for the presence of sequence variants. The results for variants whose sequences differed from the consensus sequence by each sequencing method were plotted. Five sequences of tax amplified from cell lines were examined. For PBMC from animals 12141 and 12752, 15 and 9 sequences were analyzed, respectively. For NGS, sequence variants with a cutoff of 10% variance were noted.