FIG 5.

Functionally important surface residues in UL37N as identified using universal evolutionary trace analysis (http://mammoth.bcm.tmc.edu/uet/). Individual residues were assigned relative functional importance scores based on resistance to mutations over time and clustering on the protein surface with other important residues. Residues with importance scores in the top 25% were assigned colors from red (more important) to yellow (less important). Four orientations are shown related by a 90-degree rotation around the vertical axis. Areas of the structure corresponding to the R2 region are circled in black and labeled.