TABLE 1.
Primers and probes used in this study
| Primer or probe | Targeted RNA | Sequence (5′–3′)a | Reference |
|---|---|---|---|
| RT-PCR primers | |||
| A2 (a) | AAAGCTGGGAATAGAAACTTCG | 15 | |
| JM 402 (b) | TTTATCCAGAATCTCAATCCGG | 8 | |
| JM 403 (c) | CGAAGATATTCTGGTGTAAGTCTAGTA | 8 | |
| RT-qPCR primers and probes | |||
| Forward primer | Measles virus genome | TCAGGCATACCCACTAGTGTGAA | |
| Reverse primer | TGACAGATAGCGAGTCCATAACG | ||
| TaqMan probe (reporter dye) | CATCAGAATTAAGAAAAACGTAG (VIC) | ||
| Forward primer | β-Actin | ACCGAGCGCGGCTACAG | |
| Reverse primer | CTTAATGTCACGCACGATTTCC | ||
| TaqMan probe (reporter dye) | CACCACCACGGCCGA (FAM) | ||
| Forward primer | 504-nt DI-RNA | CGGAGTTCAACCAATTAGTCCTTAA | |
| Reverse primer | TGTGCCCCCAGAATTTGC | ||
| TaqMan probe (reporter dye) | CAGGGCACTATCTAGG (FAM) | ||
| Forward primer | 1,212-nt DI-RNA | TTGCAAATAATGCCTAACCACCTA | |
| Reverse primer | ACACTGCCTACCCACGTGACT | ||
| TaqMan probe (reporter dye) | CAGGATTAGGGTTCCGGG (FAM) | ||
| Forward primer | 1,662-nt DI-RNA | TTACCTTAAAAACCCACTCACGTTT | |
| Reverse primer | AGATTGCCCCCTGAAATGG | ||
| TaqMan probe (reporter dye) | AACACAAGCAAGCACA (FAM) | ||
| Forward primer | 450-nt DI-RNA | TTATCAACTTTTTGTTCCCGGAGTA | |
| Reverse primer | ACCACCTAGGGCAGGATTAGG | ||
| TaqMan probe (reporter dye) | AGATAATTGGTTGAACTCCGGAA (FAM) | ||
| Forward primer | 942-nt DI-RNA | GAGATGAAGTTGCTGTATCTAGGG | |
| Reverse primer | AATGCCTAACCACCTAGGGCAGG | ||
| TaqMan probe (reporter dye) | TGAACTCCGGAACCC (FAM) |
a
VIC, 4,7,2′-trichloro-7-phenyl-6-carboxyfluorescein; FAM, 6-carboxyfluorescein.