FIG 1.

FGFR3 is expressed in keratinocytes with and without HPV genomes. C33A cells were transfected with FLAG-FGFR3 WT (A) or FLAG-FGFR3 K650E (B) expression plasmids. Cells were stained for immunofluorescence with FLAG (M2) and FGFR3 antibodies (ab). Red, FLAG or FGFR3 antibodies; blue, DAPI. Magnification, ×100. (C) C33A cells were transfected with a FLAG-FGFR3 WT expression plasmid and stained with FLAG (M2; red) and FGFR3 (green) antibodies. Blue, DAPI. Cells were viewed under a magnification of ×60 using confocal microscopy. (D) HaCat, NIKS, W12, HFK, CIN612-9E, and HPV-BP cells were stained with FGFR3 antibodies (red) and DAPI (blue) for immunofluorescence. Magnifications, ×100 (left panels) and ×40 (right panels).