FIG 2.

FGFR3 interacts with PV E2 but not with EGFR or EphB2. (A) HEK293TT cells were transfected with FGFR3 WT, K650E, and K508R constructs along with a BPV-1 E2 expression plasmid. BPV-1 E2 pulldown was completed with B201/B202 antibodies and blotted with FGFR3, B201, and β-actin antibodies. (B) HEK293TT cells were transfected with FGFR3 WT, K650E, and K508R constructs along with a FLAG-HPV-31 E2 expression plasmid. HPV-31 E2 pulldown was completed with M2 antibodies and blotted with FGFR3, M2, and β-actin antibodies. C33A cells were transfected with wild-type BPV-1 E2, E2R, and EGFR (C) or EphB2 (D) expression plasmids. Twenty-four hours later, cells were lysed, and BPV-1 E2 was immunoprecipitated with B201 antibodies. Immunoblotting was completed with EGFR, EphB2, and B201 antibodies. IP, immunoprecipitation.