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. 2017 Sep 27;91(20):e01066-17. doi: 10.1128/JVI.01066-17

FIG 4.

FIG 4

FGFR3 interacts with TAD of BPV-1 E2 and endogenous HPV-16 E2. (A) C33A cells were transfected with FLAG-FGFR3 WT and BPV-1 full-length, TAD (aa 1 to 216), and E2R (aa 162 to 410) constructs. BPV-1 E2 protein pulldown was completed with B201 antibodies and blotted with B201 and M2 antibodies. TAD (*) is the same size as light-chain IgG in immunoprecipitation groups. (B) Endogenous levels of HPV-16 E2 (16E2) in W12 cells were confirmed by immunoprecipitation using sheep anti-HPV-16 E2 polyclonal serum. C33A cells transfected with FLAG-HPV-16 E2 served as the positive control for the detection. HPV-16 E2 was immunoblotted with TVG-261 antibody. (C) The immune complexes of HPV-16 E2 from the W12 cells were analyzed for the presence of endogenous FGFR3 using specific antibodies. Normal sheep IgG served as the negative control.