FIG 8.

Activated FGFR3 decreases HPV replication. (A) C33A cells were transfected with pFLORI-BPV-1 (Fluc) and pRL (Rluc) constructs in the presence of FGFR3 WT, K650E, and K508R expression plasmids. Twenty-four hours later, cells were lysed, and firefly and Renilla luciferase levels were measured using Dual-Glo luciferase reagent. Firefly luciferase levels were normalized to Renilla luciferase levels. Number of samples per group, 4. (B) C33A cells were transfected with BPV-1 E1, E2, pFLORI-BPV-1 (Fluc), and pRL (Rluc) constructs in the presence of FGFR3 WT, K650E, and K508R expression plasmids. Seventy-two hours later, cells were lysed, and firefly and Renilla luciferase levels were measured using Dual-Glo luciferase reagent. Firefly luciferase levels were normalized to Renilla luciferase levels. E1 and E2 luciferase-normalized values were normalized to E2-normalized luciferase values. Values are expressed as means ± SEM (n = 4). *, P < 0.05. (C) CIN612-9E wells were transfected with pFLORI-HPV-31 (Fluc), pRL (Rluc), and WT, K650E, and K508R constructs. Seventy-two hours later, cells were lysed, and firefly and Renilla luciferase levels were measured using Dual-Glo luciferase reagent. Firefly luciferase levels were normalized to Renilla luciferase levels. Values are expressed as means ± SEM (n = 8). *, P < 0.05.