FIG 9.

FGFR3 siRNA increases HPV-31 DNA content in CIN612-9E cells. (A) CIN612-9E cells were transfected with a control, FGFR3, or EGFR siRNA. After 48 h cells were lysed (0.5% NP-40), and immunoblotting was completed with FGFR3, EGFR, and β-actin antibodies. (B) CIN612-9E cells were transfected with a control, FGFR3, or EGFR siRNA. After 48 h cells were lysed, and DNA was isolated for reverse transcription-PCR against the HPV-31 LCR and normalized to the level with the β-actin primer set. Values are expressed as means ± SEM (n = 14). *, P < 0.1.