FIG 3.

Interaction of the M2-2 protein with IRF7. (A, B, and D) HEK293T cells were transfected with the indicated plasmids. At 24 h posttransfection, the cells were lysed in lysis buffer and subjected to immunoprecipitation (IP) with anti-V5 (A) or anti-FLAG (B and D) antibody, followed by immunoblot analysis (WB) with anti-FLAG, anti-V5, or anti-myc antibody. (C) V5-TRAF6, V5-IKKα, V5-IRF7, and myc-M2-2 were synthesized in the wheat germ cell-free expression system. Then, the in vitro transcription/translation products were mixed in various combinations and subjected to IP with anti-myc antibody followed by WB with anti-V5 and anti-myc antibodies. A portion of the whole-cell lysates (A, B, and D) or the in vitro transcription/translation products (Input) (C) prepared for IP was also subjected to WB. The asterisks indicate the positions of the antibody heavy chain (AbHC).