FIG 5.

Effect of the M2-2 protein on MyD88-TRAF6-IKKα-induced homodimerization of IRF7. (A and B) HEK293T cells were transfected with the indicated plasmids. At 24 h posttransfection, the cells were suspended in Dulbecco's PBS. After addition of furimazine, luminescence and fluorescence signals were immediately detected. The BRET ratio was calculated as described in Materials and Methods. (C and D) HEK293T cells were transfected with the indicated plasmids and an internal control, pCA7-Fluc. At 36 h posttransfection, Rluc and Fluc activities were measured. Relative luciferase activity was calculated as the ratio of Fluc activity to Rluc activity. The mean values from three independent experiments are shown, with standard deviations.