FIG 6.

Effect of the M2-2 protein on IPS-1-induced activation and homodimerization of IRF7. (A) HEK293T cells were transfected with the indicated plasmids, pRL-TK, and an IFN-α6 promoter-driven Fluc reporter plasmid. At 24 h posttransfection, Fluc and Rluc activities were measured. Relative luciferase activity was calculated as the ratio of Fluc activity to Rluc activity. (B) HEK293T cells were transfected with the indicated plasmids and pCA7-Fluc. At 36 h posttransfection, Fluc and Rluc activities were measured. Relative luciferase activity was calculated as the ratio of Rluc activity to Fluc activity. The mean values from three independent experiments are shown, with standard deviations. NS3/4A, HCV NS3/4A protein.