FIG 1.

Expression of ZIKV C-prM-E by use of a codon-optimized synthetic construct. (A) A codon-optimized ZIKV C-prM-E gene was synthesized via gene synthesis technology, using the sequence available from the current outbreak in the Americas, and cloned into the pcDNA3.1 vector. (B) 293T cells were transfected with the ZIKV C-prM-E or WNV C-prM-E construct. Cells were fixed, stained with the MAB10216 and MAB8150 antibodies, and analyzed for E protein expression by fluorescence microscopy. (C) 293T cells were transfected as described above. At 48 h posttransfection, cells were radiolabeled with [³⁵S]Met/Cys. Cell lysates were immunoprecipitated with protein A beads coated with MAB10216 or anti-WNV serum, resolved by SDS-PAGE, and visualized by PhosphorImager analysis.