FIG 10.

Evidence that differentiated G144 cells support infection by JC virus. (A) (Right) Results of immunofluorescence-based experiments demonstrating that differentiated G144 cells infected with JC virus for 48 h express JCV T-ag. (Left) As a control, additional immunofluorescence studies of T-ag expression were conducted in uninfected G144 cells. (B) (Right) Additional immunofluorescence-based experiments establishing that after infection with the Mad-4 strain of JC virus for 48 h, differentiated G144 cells express the late viral protein VP1 (probed with the anti-VP1 MAb Pab 597). (Left) Results of identical immunofluorescence-based studies of uninfected G144 cells. (C) Results of a qPCR-based study demonstrating that the JCV genome is amplified following infection of differentiated G144 cells with the Mad-4 strain of JC virus for 6 days. The y axis presents the log₂-fold change between the ΔC_T values of the input and JCV DNA samples collected at the indicated times. Means (n = 3) are reported, and the error bars indicate standard deviations from the mean. *, P < 0.05.