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Efficient expression of JCV T-ag in the nuclei of G144 cells. (A) Immunofluorescence analysis revealed localization of T antigen (left) in the nucleus (indicated by DAPI [right]) of undifferentiated G144 cells following nucleofection of pCMV-JCT-ag. (B) In contrast, the results of a representative control experiment demonstrated that T-ag (left) does not accumulate in the nuclei of the Hs683 glioma cell line (indicated by DAPI [right]).
