FIG 4.

G144 cells support robust levels of JCV DNA replication, particularly when differentiated. (A) Levels of replication in both undifferentiated and differentiated G144 cells were determined by luciferase assay after 72 h (bars 2 and 6) and 96 h (bars 4 and 8). Reactions conducted in the absence of T-ag at the indicated times are represented by the odd-numbered bars. Means (n = 3) are reported, and the error bars indicate standard deviations from the mean. The asterisks indicate significant increases in replication (P < 0.05) compared with T-ag-minus (−) controls. (B) Results of a Western blot used to determine the amounts of JCV T-ag in G144 cells after 72 h (lanes 2 and 6) and 96 h (lanes 4 and 8) following nucleofection of the replication plasmids. As a loading control, an additional Western blot was performed to determine vinculin levels.