TABLE 1.
Data collection and refinement statistics
| Parameter | Valuea |
||
|---|---|---|---|
| N protein (SeMet) | N-RNA complex | N-DNA complex | |
| Data collection statistics | |||
| Wavelength (Å) | 0.97906 | 0.98000 | 1.00000 |
| Space group | P21 | P21 | P21 |
| Cell dimensions | |||
| a, b, c (Å) | 66.2, 96.0, 71.8 | 68.2, 94.7, 70.9 | 68.9, 86.5, 66.3 |
| α, β, γ (°) | 90, 113.4, 90 | 90, 112.6, 90 | 90, 112.1, 90 |
| Resolution (Å) | 50–2.70 (2.87–2.70) | 50–3.3 (3.5–3.3) | 50–3.00 (3.18–3.00) |
| Rmeas (%)b | 9.7 (76.3) | 5.2 (94.8) | 8.0 (99.6) |
| 〈I/s(I)〉 | 10.8 (2.13) | 16.5 (1.75) | 17.1 (2.38) |
| Completeness (%) | 92.3 (98.5) | 99.0 (97.8) | 99.4 (98.0) |
| No. of unique reflections | 41,185 (7,154) | 12,585 (1,985) | 14,540 (2,273) |
| Redundancy | 3.8 (3.8) | 3.7 (3.7) | 7.6 (7.6) |
| Refinement statistics | |||
| No. of reflections | 41,171 | 12,581 | 14,534 |
| Rwork/Rfree (%) | 21.04/25.52 | 28.23/31.66 | 25.05/29.13 |
| No. of atoms | |||
| Protein | 5,965 | 5,439 | 5,666 |
| RNA/DNA | 220 | 328 | |
| Average B-factors (Å2) | |||
| Protein | 77.7 | 135.4 | 103.8 |
| RNA/DNA | 227.9 | 130 | |
| RMSD from ideal | |||
| Bond length (Å) | 0.003 | 0.002 | 0.005 |
| Bond angle (°) | 0.783 | 0.531 | 0.938 |
| Ramachandran plot (%)c | |||
| Favored regions | 96.95 | 98.22 | 95.82 |
| Outliers | 0.27 | 0 | 0 |
a
Values in parentheses are for the highest-resolution shell.
b
Rmeas is the redundancy-independent Rsym value.
c
Ramachandran analysis was performed by using MolProbity in the PHENIX package.