FIG 2.

Negative staining of autophagosomes purified from cells. (A to C) Autophagosomes purified from GLR cells by membrane flotation followed by affinity purification were negatively stained using uranyl acetate for electron microscopy. (D) Representative example of autophagosomes that were purified from GLR cells and stained for NS5A by immunogold staining. Arrows highlight the gold particles that are located on the rim of the broken membrane. (E and F) Negative staining of autophagosomes purified from nutrient-starved Huh7-GFP-LC3 cells.