FIG 3.

VZV DNA region A sequence determination. (Left) Two independent samples of VZV-DEN09 DNA (sample 1, lanes 1 and 2; sample 2, lanes 5 and 6) along with duplicate samples of VZV BAC (lanes 3 and 4) were PCR amplified with primers flanking region A. All PCR products showed similar 2.8-kbp bands. M, 500-bp marker. (Right) The complete DNA sequence of the 2.8-kbp PCR product was assembled in two steps. Seq 1 obtained 702 to 976 nucleotides beginning at approximately nucleotide 565 (yellow) and 936 to 998 nucleotides ending at approximately nucleotide 3275 (green). Seq 2 completed the 738 to 1070 internal sequence (orange), with 1842 to 1869 nucleotides extending from nucleotides 712 to 738 to nucleotides 2557 to 2582 (blue).