FIG 5.

VZV-DEN09 does not express ORFs 12 or 13 and does not induce Akt phosphorylation. (A to C) Flow cytometry analyses of VZV gE expression in mock (blue)- and VZV (red)-infected cells. Human brain vascular adventitia fibroblasts (HBVAFs) (A), HFL (B), or MeWo (C) cultures were left uninfected or infected with VZV-DEN09 or VZV-DEN13 for 72 h. (D) Graphical representation of percent VZV gE⁺ cells from panels A to C. HBVAFs, gray; HFLs, red; MeWos, green. Error bars represent average percent VZV gE⁺ cells ± standard deviations from duplicates for HBVAFs. An asterisk denotes 0% of mock-infected cells expressed VZV gE⁺. (E) Cell lysates were prepared for immunoblotting and resolved on 12.5% SDS-PAGE gels. Membranes were probed for ORFs 12 and 13 and total and phospho-Akt. gE was included as an infection control, and β-actin was included as a loading control.