TABLE 2.

Oligonucleotide primers

Name	Sequence (5′–3′)	Location of 5′ enda	Application
5k-FOR	GGATTATTGCCTGCACCTCTAA	8746	PCR
5k-REV	CAGAAGCGACTACGTCTTCTAAC	8791	PCR
20k-FOR	AATGCTCAGGGCACGTAAA	24248	PCR
20k-REV	GCCGGAGTGTCCAGATATAAAC	24290	PCR
40k-FOR	CCGTTCGGCGTATCGTATTT	40775	PCR
40k-REV	TGCACGCGCTAACCATAAT	40815	PCR
60k-FOR	CCCGAAGAGAGTTTGGATTACC	64941	PCR
60k-REV	CGTGTGGGCCATGGATAAA	64988	PCR
80k-FOR	GTTGTGAAGCGTTCCAATTCC	80249	PCR
80k-REV	ACGTCATCAGGCCGTTATTC	80291	PCR
100k-FOR	CCTTGGTTGCATCTCCAAGTA	100574	PCR
100k-REV	CCTCTACGGGAGCAAACTAAAG	100619	PCR
Area A For	CCCTTACTGCTGTCGTGGTCGCC	495	PCR
Area A Rev	GGACTCACGTATTGCCCGCGCG	3344	PCR
Area A-For first seq	GTTGCCGTTTTTCCCGAGGA	501	Sequence
Area A-Rev first seq	CGTGAGAGAGTCATACGTG	3319	Sequence
Area A-For second seq	CTCTGTTTATGGCGGGGTAA	1213	Sequence
Area A-Rev second seq	TGTTAGGGTTGGGAGAGTGG	2383	Sequence
Area B For	AGCGCGGAGTTCTTTGG	18476	PCR
Area B seq For	CGCAATGTTTTCTCGGTTTGCG	16012	Sequence
Area B seq Rev	CTGAAGTTCGCCGGTTAACG	18707	Sequence
Area B Rev	GCGTAGCCATGCATTTACTAC	16198	PCR
Area C/C′-FOR	GGAAGTGCGAACGGGTTTA	117892	PCR
Area C/C′-REV	CGAAGAACTTCATATCTGAGGCA	117935	PCR

^aLocation based on archive VZV strain 32 passage 72 DNA sequence (accession no. DQ479963.1).