TABLE 7.
Target genes and primers used for cloning in this studya
| Target gene | Primer | Purpose of primer | Sequence (position-5′–3′ sequence-position) |
|---|---|---|---|
| mCherry | MP1 | Forward primer for mCherry | CTCGAGGGATCCTCTAGAAAGCTTCACACCGCATACAGGTGGCAC |
| MP2 | Reverse primer for mCherry | GAATTCGGTACCAGATCTCCCGGGTTAGCCCTCCCACACATAAC | |
| M005L/R | MP3 | Forward primer for FS1 | 155083-CCGCTCGAGCGGGTCGGAGGACGTACGAAC-155100b |
| MP4 | Reverse primer for FS1 | 155363-GCTCTAGAGCCCTCGTCCGGGGTTATATC-155381 | |
| MP5 | Forward primer for FS2 | 156861-GAAGATCTTCCCTATTTGGTAGGTCCTATCC-156881 | |
| MP6 | Reverse primer for FS2 | 157153-GGAATTCCATATGGAATTCCCCATGGTTCCTACCCTC-157169 | |
| M012L | MP7 | Forward primer for FS1 | 13800-CGGGATCCCGTGACTTCCGAGAACATAGCCGCGTC-13824 |
| MP8 | Reverse primer for FS1 | 14104-CCCAAGCTTGGGCGTCGATGATGTCTCGTTTAAAGACG-14129 | |
| MP9 | Forward primer for FS2 | 14620-GAAGATCTTCGACATACACCTCGTTGCTTCTTCGG-14644 | |
| MP10 | Reverse primer for FS2 | 14883-CGGAATTCCGCGATGGAGGACCTATACATCGAACG-14907 | |
| M014L | MP11 | Forward primer for FS1 | 14847-CGGGATCCCGGCCAGGTCTATTCTGTCGATC-14867 |
| MP12 | Reverse primer for FS1 | 15109-CCCAAGCTTGGGGAGTCTCTTGTCGTTCTGGGAG-15130 | |
| MP13 | Forward primer for FS2 | 16676-GAAGATCTTCGGTAATCGCTAACGCGTTTTTCG-16698 | |
| Mp14 | Reverse primer for FS2 | 16919-CGGAATTCCGCATACACCGTCCGAGGAACGAG-16940 | |
| M153R | MP15 | Forward primer for FS1 | 148269-CGGGATCCCGCGGAAACGGGACACAACGTAACG-148291 |
| MP16 | Reverse primer for FS1 | 148505-CCCAAGCTTGGGCAGTAGCCATGTTTACACAACC-148526 | |
| MP17 | Forward primer for FS2 | 149238-GAAGATCTTCGGTATCTCGTTTGTCTATAACAAAGATCG-149266 | |
| MP18 | Reverse primer for FS2 | 149479-CGGAATTCCGCGGCAATCAACTTCCCGACTCG-149500 | |
| M156R | MP19 | Forward primer for FS1 | 149948-CGGGATCCGTAACAACACGTGTGTCGTAGCG-149970 |
| MP20 | Reverse primer for FS1 | 150178-CCCAAGCTTGGGGTGTCTTGCCTGTAGTTCAGAT-150199 | |
| MP21 | Forward primer for FS2 | 150358-GAAGATCTTCGGACTATGGACGTTCCGATACGTC-150381 | |
| MP22 | Reverse primer for FS2 | 150597-CGGAATTCCGCACGCGTTGCACGGAGGTATTG-150618 |
a
Restriction sites in primers are underlined; the 2 or 3 bases on either side of the restriction site were added as spacers.
b
Sequence positions are for the SLS genome (GenBank accession number JX565574).