Figure 2.

CB1 positively correlated with M1-type markers (CD86) and blockade of CB1 especially reduced the proportion of M1-type bone marrow-derived monocytes/macrophages (BMMs) in injured liver. The correlation between mRNA levels of CB1 and CD86 and arginase1 (A). M1 markers were measured by RT-qPCR, western blot, and CBA. After CCl₄ administration for 4 weeks, the mRNA levels of M1 type markers were measured (B). Representative scatter plots of CBA are shown (C). Three bead populations represented MIP-1β, TNF, and IL-6 based on allophycocyanin fluorescence intensity from high to low. The number presented the concentration of these proteins. The expression of inducible nitric oxide synthase was measured by western blot (D). Among macrophages of mouse liver that were isolated, total M1-type macrophages (CD86⁺) (E), M1-type cell of BM origin (EGFP⁺CD86⁺) [(F) left] and M1 type of Kupffer cell (EGFP⁻CD86⁺) [(F) left] were analyzed by fluorescence-activated cell sorting (FACS). Quantification of FACS for cell proportion [(E) right and (F) right]. *P < 0.05 compared with olive oil plus vehicle group, ^#P < 0.05 compared with CCl₄ plus vehicle group (n = 6/group).