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. 2017 Sep 28;8:1214. doi: 10.3389/fimmu.2017.01214

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Copyright © 2017 Tian, Li, Yang, Chang, Fan, Ji, Xie, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CB1-siRNA in vivo reduced the polarization of bone marrow-derived monocytes/macrophages (BMMs) toward M1 phenotype. Effectiveness of CB1-siRNA in vivo was measured by RT-qPCR (A). M1 markers were measured by RT-qPCR (A), western blot (C), and CBA (B). Among macrophages of mouse liver that were isolated, total M1-type macrophages (D), M1 type of BM origin [(E) left], and M1 type of Kupffer cell [(E) left] were analyzed by fluorescence-activated cell sorting (FACS). Quantification of FACS for cell proportion [(D) right and (E) right]. *P < 0.05 compared with olive oil plus vehicle group, ^#P < 0.05 compared with CCl₄ plus vehicle group (n = 6/group).