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. 2017 Sep 28;8:1214. doi: 10.3389/fimmu.2017.01214

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Copyright © 2017 Tian, Li, Yang, Chang, Fan, Ji, Xie, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Activation of CB1 promotes M1 marker expressions in bone marrow-derived monocytes/macrophages (BMMs). BMMs were exposed to ACEA for different times. The mRNA levels of M1 markers were measured (A). BMMs pretreated with 10 µmol/L AM281 or CB1 RNA interference was exposed to ACEA for 5 h. M1 markers were measured by RT-qPCR (B,C), western blot (E,F), and CBA (D). *P < 0.05 compared with control, ^#P < 0.05 compared with the same treated group without inhibitors (n = 4).