Fig. 2. Rupture forces of the HR–cy couple. (A) Representative experiments showing the force extension curves of DNA constructs harbouring at their ends the HR–cy (red, 50 nm s–1, green 200 nm s–1) or HR–cyCH3 (blue, 50 nm s–1) couples. The mechanical unzipping of DNA constructs without the HR component is shown in grey. Four independent curves are shown in each case (see ESI† for additional curves). The positions of the GCC clusters are revealed as peaks in the unzipping pattern. Arrows indicate the force and position of the rupture events. (B) Average rupture forces for DNA constructs without the HR–cy couple (grey), with the HR–cy couple (red, 50 nm s–1, green 200 nm s–1) and with the HR–cyCH3 couple (blue, 50 nm s–1). Error shows standard deviations; see the main text for populations. Data were taken at 100 Hz (22 ± 1 °C).
