Table 8.

Insulin levels (μIU/mL) according to amount of supplement fed

Item	Treatmenta				SEM	P-valueb
	3.0 kg	1.5 kg	1.0 kg	0.0 kg		L	Q	C
Pre-supplementation	1.68	1.50	1.18	1.13	0.43	0.13	0.79	0.63
Post-supplementation	2.20	1.48	1.33	1.25	0.38	0.03	0.21	0.13

^aTreatments: 3.0 kg – cows received 3.0 kg of concentrate daily; 1.5 kg – cows received 1.5 kg of concentrate daily; 1.0 kg – cows received 1.0 kg of concentrate daily; and 0.0 kg – no concentrate supplement was fed

^bL, Q and C – effects of linear, quadratic and cubic order of level of supplementation