Fig. 1.

A small group of miRNAs have MREs within the 3’UTRs of TARDBP, RGNEF and FUS/TLS. a 3’RACE PCR identified the 3’UTRs of TARDBP, FUS/TLS and RGNEF that are expressed in the human spinal cord tissue within three different control samples. One isoform of both TARDBP and FUS/TLS were identified with a length of 1398 and 150 bases, respectively. Two isoforms of RGNEF, which we have termed RGNEF-short (RGNEF-S) and RGNEF-long (RGNEF-L) 3’UTRs, were identified in all three subjects running at 177 and 981 bases, respectively. Bands in figures appear higher than actual 3’UTR size, as primers were designed upstream from stop codon. b Five miRNAs were identified to have binding sites within the TARDBP, FUS/TLS and RGNEF mRNA 3’UTRs. MiR-548d-3p in previous work has shown to have no dysregulation in sALS, and thus, four miRNAs (outlined in the black box) went under further study. c Schematic of all TARDBP, FUS/TLS and RGNEF 3’UTRs identified within human spinal cord, and location of MREs for miRNA candidates