Fig. 5.

MiR-b2122 and miR-194 directly interact with their 3’UTR targets. HEK293T cells were co-transfected with either the pmirGLO plasmid containing the wild-type 3’UTR, or the 3’UTR mutant, and either with or without the miRNA of interest. Mutations within the MRE of miR-b2122 in the TARDBP, FUS/TLS, and RGNEF 3’UTRs, and the MRE of miR-194 in the TARDBP and FUS/TLS 3’UTRs abolished each miRNAs ability to reduce firefly activity. Firefly was normalized to renilla, and then further normalized to the effect of each miRNA on the pmirGLO vector to determine the miRNAs exact effect on the 3’UTR. Data is expressed as sample mean ± SEM, and significance was determined using a student’s t-test (*** = p < 0.001, ** = p < 0.01, * = p < 0.05)