Fig. 6.

MiR-b2122 regulates mRNA expression of TARDBP, FUS/TLS and RGNEF in a human neuronal cell line. SH-SY5Y cells were transfected individually with miR-194 or miR-b2122, or co-transfected with miR-194 or miR-b2122, and their anti-miRs. Transfection of let-7a was used as a negative control. a Transfection of miR-b2122 significantly down-regulates the mRNA levels of TARDBP, FUS/TLS and RGNEF, while co-transfection of miR-b2122 with its anti-miR led to a recovery in the mRNA levels. Transfection of the anti-miR alone lead to increased mRNA expression of all three RNA-binding proteins (b) Transfection of miR-194 alone resulted in reduction of TARDBP and FUS/TLS, which was abolished when it was co-transfected with its anti-miR. No effect was observed on RGNEF when miR-194 and/or its anti-miR were transfected. Data was expressed as the mean ± SEM, and significance was determined using a one-way ANOVA followed by a Tukey’s post-hoc. (*** = p < 0.001, ** = p < 0.01 * = p < 0.05)