Figure 1.

MMP-8 reduced the invasion of OTSCC cells. MMP8 gene expression and β-actin (ACTB) were measured from the same RT reaction samples. Overexpression of pro-MMP-8 was routinely confirmed by western blot from 20 μg of conditioned media (representative result shown) (A). Organotypic 3D leiomyoma tissues were used to analyse cell invasion (B). The areas of invasive cytokeratin positive cells were quantified with Fiji software (C). *P<0.05, **P<0.01, n.s.=not significant. PCR was performed using samples from three different individual experiments (representative results shown). The myoma experiment was performed with duplicate leiomyoma disks per culture condition; three sections per disk were used in analyses.