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. 2017 Aug 3;117(7):1007–1016. doi: 10.1038/bjc.2017.249

Figure 3.

Figure 3

MMP-8 affected the expression levels of various proteinases. MMP-8+ HSC-3 and SCC-25 cells expressed less MMP-1 than controls cells analysed by western blot from 20 μg of conditioned media (A). The level of MMP-9 (but not MMP-2) was increased in conditioned media of MMP-8+ HSC-3 and SCC-25 cells analysed by zymography (B). Cathepsin-K (CTSK) mRNA levels were analysed from MMP-8+ and control HSC-3 cells, and MMP8 mRNA levels from cathepsin K-silenced and control cells using real-time quantitative PCR (qRT–PCR). The Y axis shows the ratio of CTSK or MMP-8 mRNA levels to the peptidylprolyl isomerase (PPIA) mRNA level (with standard deviations) (C). The level of MMP-14 was not changed in MMP-8+ HSC-3 cells as analysed by western blot from 40 μg of cell protein extract (D). MMP-1 western blots and gelatin zymography were performed twice from two different individual experiments with HSC-3 and SCC-25 cells and qRT–PCRs in triplicates from two individual experiments. For MMP-14 western blot, three individual experiments of HSC-3 cells were analysed. **P<0.01, ***P<0.001.