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. 2017 Aug 3;117(7):1007–1016. doi: 10.1038/bjc.2017.249

Figure 4.

Figure 4

MMP-8 overexpression blocked TGF-β1-induced VEGF-C expression and low MMP-8/high VEGF-C associated with poor prognosis of OTSCC patients. VEGF-C was analysed by western blot from equal volumes of conditioned media (three individual experiments with different cell passages) with and without 10 ng ml–1 TGF-β1. Representative samples are shown. The values from densitometer analyses (Fiji) were adjusted to the protein concentration of each media (A). VEGF-C and MMP-8 in OTSCC samples (n=57) were detected by immunohistochemistry. The combined MMP-8−/VEGF-C+-status was highly predictive for poor cancer-specific survival as analysed by Kaplan–Meier method and compared with the other combinations (MMP-8+/VEGF-C+, MMP-8+/VEGF-C− or MMP-8−/VEGF-C−) using the log-rank test (P<0.001) (B). Multivariate analysis was done using backward stepwise selection of variables, and a P-value of 0.05 was adopted as the limit for inclusion of a covariate. HRs, hazard ratios; CI, 95% confidence interval (C). ***P<0.001, log-rank test.