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. 2017 Aug 8;117(7):1036–1047. doi: 10.1038/bjc.2017.255

Figure 8.

Figure 8

miR-29a/b/c and CDC42 siRNA suppress the activation of CDC42-PAK pathway. (A–C) Left: western blot analyses of CDC42, p-PAK1/2/3, p-LIMK1/2 and p-cofilin in U87MG and U251 cells untreated (Mock) and transfected with scrambled control sequence (Scr), miR-29a/b/c mimics, miR-29a/b/c mimic mixture (miR-29s) or CDC42 siRNA (si-CDC42) and CDC42 expression plasmid plus miR-29s (miR-29s+CDC42) or si-CDC42 (si-CDC42+CDC42). Right: Comparisons among groups of CDC42, p-PAK1/2/3, p-LIMK1/2 and p-cofilin levels in the cells as indicated. Their relative levels were normalised against β-actin protein. All experiments were performed at least in triplicate and the data in A–C are presented as the mean±s.d. *P<0.05; **P<0.01; ***P<0.001. (D) Schematic illustration of the molecular pathway by which miR-29a/b/c inhibit glioma cell migration and invasion.