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. 2017 Aug 22;117(7):954–964. doi: 10.1038/bjc.2017.277

Figure 1.

Figure 1

The RNAi synthetic lethal screen. (A) Schema of siRNA kinome screen. (B) Ranked mean ΔZ-scores, kinase hit threshold ⩽−2 (n=2). (C) Quantification of percentage survival of FBXW7+/+ (black) and −/− (grey) HCT116 cells 5 days post transfection with 8 individual GAK siRNAs from the deconvoluted siGENOME (1–4) and ON-TARGET plus (5–8) SMARTpools (n=3); mean±s.e.m. Paired T-test, *P<0.05, **P<0.01, ***P<0.001; NS, not significant. (D) Immunoblot showing GAK and GAPDH levels in FBXW7+/+ and −/− cells 72 h following GAK RNAi.