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. 2017 Aug 22;117(7):1048–1062. doi: 10.1038/bjc.2017.286

Figure 3.

Figure 3

Analysis of pH2AX and RAD51 foci formation. (A) Inmunofluorescence analysis of pH2AX induction after gamma-ray irradiation (10 Gy) was assessed in CL-V4B RAD51C WT, CL-V4B RAD51C.p.Arg312Trp, CL-V4B EV and V79B EV cells. (B) Percentage of cells with pH2AX foci is shown. (C) RAD51 foci formation was evaluated in V79B EV, CL-V4B EV, CL-V4B RAD51C WT and CL-V4B RAD51C.p.Arg312Trp cells after gamma-ray irradiation (10 Gy). (D) Percentage of cells with at least one RAD51 foci is presented. Non-irradiated cells (–) were included as controls. Representative images (40 ×) of each experiment are exposed. * P<0.05; ** P<0.01; *** P<0.001. Values correspond to the mean±s.e.m. of three independent experiments. A full colour version of this figure is available at the British Journal of Cancer journal online.