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The effects of knockdown of STMN1 and FOXM1 on TKI-induced CSC enrichment and drug resistance in NSCLC cells. Colony formation (A) and sphere formation (B) were assessed in TKI-pretreated NCI-H460 cells after knockdown of STMN1 or FOXM1 by siRNA (50 nM). (C) The sensitivity to TKIs was assessed by RTCA proliferation assays in TKI-pretreated NCI-H460 cells after single knockdown of STMN1 and FOXM1 by siRNA (50 nM). All error bars are s.e.m. *P<0.05, compared with the control.
