Fig. 3.

Bach2 competes with AP-1 for binding to the IL-2 proximal promoter. (A to D) Jurkat T cells were transfected with Bach2-and/or AP-1-expressing vectors, along with reporter constructs, as indicated. The cells were cultured in the presence (B and D) or absence (A and C) of PMA/ionomycin (P/I) for 24 hours and analyzed using luciferase assays. (E) Jurkat T cells were transfected with pcDNA3-2×FLAG-Bach2 alone or together with AP-1-expressing vectors and assayed by ChIP-qPCR methods. The data are representative of three independent experiments. *P < 0.05 and **P < 0.01 by Student’s t-test.