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. 2017 Jan 20;2(1):49–58. doi: 10.1016/j.synbio.2016.12.002

Fig. 2.

Fig. 2

Representative SDS-PAGE gel Tris-Acetate pre-cast gel (7% acrylamide, Coomassie R-350 staining) demonstrating the use of CRM197 to form higher molecular weight PnPS-CRM conjugate using in vacuo glycation; (a) reference ladder in kDa, (b) activated PS and free protein mixture before conjugation with evident free CRM197 band near 58 kDa, and (c) PnPS-CRM197 conjugate, with low to no free protein as evidenced by the absence of bands below the 66 kDa ladder line. 1.5 μg samples were loaded into each respective lane.