Figure 5.

Aloperine regulates PI3K/Akt/mTOR signaling in vitro and in vivo. (a) Western blotting analysis of PI3K/Akt/mTOR in Jurkat cells after 24 h treatment with 0.5 mM aloperine with or without different inhibitors (10 nM wortmannin (PI3K inhibitor), 4 μM LB-100 (PP2A inhibitor), or 4 μM perifosine (p-Akt inhibitor)). The data are shown as the mean ± SEM from three independent experiments. (b) Western blotting analysis of PI3K/Akt/mTOR in the mouse colon. (c) Western blotting analysis of protein phosphatase 2A (PP2A) in the mouse colon. The data are shown as the mean ± SEM of 6 mice analyzed for each group. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001.