Table 4.
Method | Throughput | Cost | Transcript abundance | Produce absolute results | Require a priori knowledge | Accuracy of results |
---|---|---|---|---|---|---|
RNA-sequencing | High | Low | Low | Yes | No | High |
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DNA microarrays | High | Low | High | No | Yes | Depends on transcript abundance |
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Genomic tiling arrays | High | Low | Low | Yes | No | High |
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Tissue microarrays | Very high | Low | Low | No | Yes | High |
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Northern blotting | Low | Low | High | No | Yes | Low |